H. Saify Nabiabad; M. Naierifard; M. Sharifani; Kh. Piri
Abstract
The tuberose (Polianthes tuberosa L.) is an ornamental plant related to the agaves, planted around the word. The main purpose of this study was to investigation of various concentrations of Thymus vulgaris L., Rosmarinus officinalis L., and benzyl adenine on longevity and durability of tuberose cut flowers. ...
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The tuberose (Polianthes tuberosa L.) is an ornamental plant related to the agaves, planted around the word. The main purpose of this study was to investigation of various concentrations of Thymus vulgaris L., Rosmarinus officinalis L., and benzyl adenine on longevity and durability of tuberose cut flowers. After extracting herbal extract and GC analysis, cut flowers were transferred to vessels containing preservative solutions. Then flowers were located in the controlled room. A factorial experiment in randomized complete block design with three replications was carried out in this research. Design include effects of treatments on quality of flowers, percent of total dissolved solids, water absorbance, stem bending, flower diameter, fresh weight and dry weight. GC analysis indicated that there were some compounds in the extracts increasing the shelf life of cut flowers. The most longevity (13.75 days) was related to treatment of interaction of thymus extract (10%) and 50mg/l benzyl adenine. In conclusion, treatments of benzyl adenine and thymus extract inhibited the growth of microorganisms in preservative solutions that resulted in increased post-harvest longevity.
K. Pirian; Kh. Piri; J. Sohrabipour; S. Tamadoni Jahromi; R. Rabiei
Abstract
Nutritional, pharmaceutical, and healthcare properties of macroalgae are the most investigated subjects in macroalgae. In this study, nutritional and physicochemical properties of two important green macroalgae, Ulva intestinalis and Ulva linza, collected from the Persian Gulf, were investigated. In ...
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Nutritional, pharmaceutical, and healthcare properties of macroalgae are the most investigated subjects in macroalgae. In this study, nutritional and physicochemical properties of two important green macroalgae, Ulva intestinalis and Ulva linza, collected from the Persian Gulf, were investigated. In all analyses, we followed the reference methods with some modification. Results revealed that U. intestinalis contained higher protein content (15.79%) as compared with U. linza (9.90%) while U. linza had higher content of lipid (2.30%), ash (26.66%), phenol (2.16 mgGA/g) and flavonoid (11.7 mgQE/g) as compared with U. intestinalis with 1.98% lipid 22.50% ash, 1.02 mgGA/g phenol and 8.2 mgQE/g flavonoid. The two species did not show any significant differences about all analyzed compositions (P<0.05) except lipid content. In physicochemical analyses, U. intestinalis showed higher water holding capacity (17.5 g/g), swelling capacity (9.5 mL/g), and oil holding capacity (5.5 g/g) as compared with U. linza with water holding capacity of 8.7 g/g, swelling capacity of 5.7 mL/g and oil holding capacity of 2.6 g/g. The difference between physicochemical properties of two species were all statically significant (P<0.05). The study showed that the two algae species investigated with high protein content could be considered as valuable plant sources for direct consumption as human food and due to the unique physicochemical properties, the two species could be used as ingredients to improve the structure and nutritional values of the food products in food industries.
A. Badrhadad; Kh. Piri; K. Mansouri
Abstract
Previous studies have shown an anti-tumoral effect for different species of plant having similar component with Elaeagnus angustifolia L.and Nepeta crispa L. on cancer cells. This study was performed to evaluate the anti-tumural effects of Elagnus angustifolia and Nepeta crispa extracts on leukemia cancer ...
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Previous studies have shown an anti-tumoral effect for different species of plant having similar component with Elaeagnus angustifolia L.and Nepeta crispa L. on cancer cells. This study was performed to evaluate the anti-tumural effects of Elagnus angustifolia and Nepeta crispa extracts on leukemia cancer cells. Cancer cells, grown in DMEM/F12 culture medium, were supplemented with fetal calf serum, 100U/ml penicillin and 100μg/ml streptomycint, then incubation at 37°C in a 5% CO2. The fractions of Hexane, ethyl acetate, chloroform and aquesse at 10-160 μg/ml concentration were applied on K562 leukemic cells, and their effects were evaluated on proliferation and viability of cells by cell counter and LDH kits, respectively. Result showed that chloroform (chf), ethylacetate (EA) fractions of Elaeagnus angustifolia and Nepeta crispa showed an important cytotoxic effect on K562 cells. Isolated extracts from E. angustifolia flower and N. crispa caused a significant decrease in lukemia cancer cell growth. Therefore, this plant could be candidate for therapeutic or preventive activity against cancer-related disorders. Isolation and purification of effective compound/s from this extract and determination of their mechanisms of action are suggested.
K. Pirian; Kh. Piri
Abstract
Portulaca oleracea L. as a medicinal plant, having valuable secondary metabolites, such as noradrenaline, dopamine and omega-3, is used as anti-cancer, antioxidant, and blood purifier factor. Callus inducted from hairy roots of some medicinal plants are used to increase the production of secondary metabolites, ...
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Portulaca oleracea L. as a medicinal plant, having valuable secondary metabolites, such as noradrenaline, dopamine and omega-3, is used as anti-cancer, antioxidant, and blood purifier factor. Callus inducted from hairy roots of some medicinal plants are used to increase the production of secondary metabolites, cell suspension culture, protoplast culture, induction of embryonic callus and gene transfer agents. In this experiment, various concentrations of two hormones of BA and 2,4-D were examined on transgenic hairy roots of P. oleracea to produce callus. Hairy roots transgenic of P. oleracea were induced by Agrobacterium rhizogene, 15834 strain. Hairy roots produced by Agrobacterium rhizogenes15834 strain, were transferred to 1/2MS medium containing different concentrations of BA and 2,4-D hormones. BA and 2,4-D hormones were used, both at three levels of 0, 0.5 and 1 mg per liter and in three replications. Our results indicated that BA and 2,4-D hormones alone as well as without hormone treatment (control) did not show any callus formation. The treatments containing different ratios of BA and 2,4-D hormones, caused callus formation in varying degrees. The medium containing 1mg/L BA and 1 mg/L of 2,4-D hormones showed the higher production of callus.
